An Aptamer Affinity Column for Extraction of Four Aminoglycoside Antibiotics from Milk

This article introduces the aptamer affinity column (AAC) with nucleic acid as an ligand for extraction of four aminoglycoside antibiotics (AGs). The AAC was prepared by loading functionalized Sepharose into column, which conjugated covalent binding between NHS-activated and amino-modified aptamers a coupling time 2 h. After sample solution flowed through AAC, AGs were retained because aptamer, then eluted analyzed UPLC-MS/MS. Under optimized conditions, maximum adsorption on could reach 8.0 μg. Moreover, proposed be reused more than 20 times. resultant that successfully applied in enrichment purification milk good recovery results range 83.3–98.8% obtained (with RSD 0.6–5.8%). recognition multi-target exhibited effects, showing great application potential targets their related aptamers.